Answers based on DNA technology
To answer the question whether pathogens can cause problems if the dredging sludge is reused, it is important to determine whether it contains pathogens that are still viable. Normally, culturing methods are used for such issues. However, culturing of sludge samples is very difficult. Therefore we use DNA-technology. Usually, we measure all present species, both alive and dead with DNA-technology. As the dead material is not relevant in this case, we have looked for a method that only determines the living fraction. The innovative DNA-analysis method vPCR (viable PCR) is mostly applied to analyse water samples. We have adjusted this method so it can be applied to sludge samples as well. Due to this development, we can now apply the method to various solid matrices (soil, sludge, pulp).
For this reason, we were able to determine if, and how many viable pathogens were present in the sludge. During this pilot research, monitoring was conducted by analysing sludge samples at various dates using the vPCR method.