Our laboratory

The work carried out in our laboratory focuses on understanding the negative impact that micro‑organisms can have on operational environments. This includes research into MIC (microbiologically influenced corrosion), biofilm formation and the presence of pathogenic micro‑organisms.

MIC corrosion and biofilms

In our laboratory, we investigate the micro‑organisms responsible for biofilm formation and MIC-corrosion. We work with a wide range of sample types, including water, soil, surfaces and corrosion products. In addition to identifying micro‑organisms, we also examine which environmental factors promote undesirable microbial activity. By processing the analysis results using our in‑house data processing tool, we map these processes clearly, enabling clients to gain insight into risks such as material loss, blockages and process disruptions.

Pathogen detection

In addition to MIC testing and investigations into microbial blockages, we also offer pathogen detection services. In any operational environment, there is a risk of unwanted microbial intruders that can cause significant problems. We carry out targeted assessments to determine whether pathogenic micro‑organisms are present and whether they pose a risk to processes or health.By using DNA/RNA‑based analyses, you are no longer dependent on the cultivability of pathogens, as is the case with traditional methods. This enables a broader diagnosis and offers deeper insight. With NGS, it is also possible to look beyond standard pathogens and gain a clearer understanding of the wider microbial community. In the event of a suspected contamination, you will quickly receive clarity and practical advice on the appropriate measures to take.

Diagnostic tools for microbial analysis

Depending on the research question, we use various diagnostic tools to analyse microbial activity.

qPCR-analysis

qPCR is a quantitative method for measuring DNA and RNA, allowing you to determine the abundance of a pre‑selected target organism or process. Unlike culture‑based methods, qPCR also detects non‑cultivable micro‑organisms (approximately 99% of what occurs in nature). This provides a realistic and reliable picture of microbial activity in pipes, installations and water systems. A key advantage is that samples can be fixed in the field, ensuring that the microbiological composition remains unchanged during transport.

vPCR – analysis of viable micro‑organisms

vPCR is similar to qPCR, but focuses specifically on viable micro‑organisms: cells that are still active or capable of becoming active again, such as spores. The method is always carried out in conjunction with qPCR and reveals which proportion of the micro‑organisms in a sample is still truly ‘active’. Because vPCR — just like qPCR — analyses genetic material, you are not limited to what can be cultured. However, samples for vPCR must be supplied fresh, as they cannot be fixed.

Next-generation sequencing (NGS)

NGS is a tool we use to identify all the micro‑organisms present in a sample, including species that are not yet known. It does not provide exact quantities, but it does show which groups are more or less abundant. Using 16S amplicon sequencing, we map the microbial population and gain insight into the role these micro‑organisms play in a process. The results can be used, for example, to develop qPCR analyses for monitoring. With total RNA sequencing, we can even analyse all organisms and their activity without having to select a target in advance.

Next-generation sequencing with the Illumina MiSeq i100

For our NGS analyses, we use the Illumina MiSeq i100, one of the latest compact NGS platforms. This system delivers high‑quality data, reliable DNA profiles and fast turnaround times. As a result, we can provide clients with in‑depth microbial analyses and well‑founded reports in a timely manner.

Meer over Diagnostische Tools

Is recycling overflow dredging sludge safe?

Overflow dredging sludge is sludge that is removed from ditches and canals in the vicinity of a sewage overflow. Often, this sludge is polluted because in case of heavy rainfall the sewage water and sludge will flow into the overflow simultaneously. Annually, the cost of the removal and transportation of dredging sludge that is potentially polluted is very high. Therefore, the Water Board Noorderzijlvest, commissioned Bioclear earth in cooperation with Microbial Analysis to investigate the safety of recycling the sludge in a sustainable way. For the water board it is important to know how quickly pathogenic microorganisms and drug residues disappear from the sludge.

Sampling kits for microbiological testing

To ensure a reliable microbiological analysis, you can easily order a sampling kit from us. Within one working day, you will receive all the necessary materials and a clear sampling protocol. The kit can be returned to us without refrigeration.

Once we receive the sample, you will receive an analysis report by email within ten working days. In urgent cases, we can provide a report within 48 hours for an additional charge.

Would you prefer not to take the samples yourself? Our specialists can carry out the sampling entirely on your behalf.